Coding

Part:BBa_K2686000:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)


Encapsulin with HexaHistidine insert and C-terminal OT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

The HexaHistidine insert was codon optimized for expression in E. coli, the same was done for the OT1 peptide at the part's C-terminus.

Source

Stems from K2686005. A OT1 peptide was attached to demonstrate the successful insertion of antigen sequences onto the encapsulin protein (Choi et al., 2016).

References

Choi, B., Moon, H., Hong, S., Shin, C., Do, Y., Ryu, S. and Kang, S. (2016). Effective Delivery of Antigen–Encapsulin Nanoparticle Fusions to Dendritic Cells Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection. ACS Nano, 10(8), pp.7339-7350.